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1. Take the pictures without significant shadow
2.Open ImageJ,File--Open--select the object image
3.Analyze-set Measurements
the objective is to measure the porosity(V_pore/V=(hArea_all-hArea_DM)/hArea_all)
so the Area and Are fraction are mandatory to select
4.Wand tool(single-click)-click on a random pore in the image
double-click the wand tool and then adjust the tolerance to fit the pore
5.Transfer to grey-scale map
Make sure the options are set before make binary(default settings)
6.Set threshold
Image--adjust-threshold
7.Analyze-Measure
use "Rectangle" to measure local porosity
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